toxicity assessment of herbal medicine using zebrafish embryos

This study provides the pieces of evidence for the reduced toxicity of E. kansui treated with vinegar [33]. The toxic effects of these plant extracts were compared using in vitro cytotoxicity assay using 1.1B4 (human-derived pancreatic β-cell line), 3T3-L1 (mouse-derived adipocyte like cells), and WRL-68 cell (human hepatic cell line) types. In spite of the widely reported therapeutic applications of C. longa , research on its safety and teratogenic effects on zebrafish embryos and larvae is still limited. It is recommended for abdominal pain, headache, menstrual problems, vomiting, and diarrhea. Small size, short life cycle, and high fecundity favour its laboratory uses as a test organism [9]. Furthermore, this model will allow understanding the molecular mechanism underlying the toxicity of the natural products. It was observed that the survival rate of zebrafish embryo decreased as the … 236: fish embryo acute toxicity (FET) test,” in, C. Parng, W. L. Seng, C. Semino, and P. McGrath, “Zebrafish: a preclinical model for drug screening,”, M. W. Hung, Z. J. Zhang, S. Li et al., “From omics to drug metabolism and high content screen of natural product in zebrafish: a new model for discovery of neuroactive compound,”, D. Moher, A. Liberati, J. Tetzlaff, and D. G. Altman, “Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement,”, S. D. Perera, U. The LC50, LC10, MNLC (maximum nonlethal concentration), and EC50 values were calculated as 1.76 μM, 0.8 μM, 0.5 μM, and 0.723 μM, respectively. The developmental toxicity of EE were investigated using zebrafish embryo model. State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. At 96 and 120 hpf, kink and bend tail were observed, respectively, in embryos treated with 62.5 μg/mL. Hence, the zebrafish assay was employed to assess the development toxicities of flowers of safflowers. The dried root of Euphorbia kansui is a traditional Chinese medicine used against cancer, pancreatitis, and intestinal obstruction. Both adults and embryos of the zebrafish are used as laboratory models [11]. Test organisms range from invertebrates such as brine shrimp to mammals such as mice, rats, guinea pigs, and rabbits [4]. Particularly, the zebrafish model was effective in detecting nephrotoxicity. Malformations such as oedema, crooked trunk, and abnormal morphogenesis of some organs, such as statolith, swimming bladder, and yolk syncytium, were reported in embryo treated with 0.1–1.5 μg/mL emodin [37]. Both cardiovascular and hepatic toxicities were observed for the fractions extracted using n-butyl ethanol and dichloromethane [32]. Moreover, after treatment with Tan-IIA ≥ 6 μM, the time it took for the embryos to mature into fish was longer than that for the control [43]. [31]. Recently, herbal medicine has gained resurgent interest in the Western world due to the evidence of better patient tolerance and holistic treatment approach. Full-text available. The TC50 (teratogenic effect) of 2 hpf embryos was much lower (1.67 ± 0.23 μg/mL) compared with that of 10 hpf and 24 hpf (TC50-10 μg/mL) and 48 hpf (TC50-20 μg/mL). Zebrafish AB strain and the Tg(cmlc2:EGFP), Tg(L-FABP:EGFP), Tg(Lyz:EGFP), and Tg(Vmat:GFP) transgenic zebrafish lines were used for lethality and other specific organ toxicity studies. In 5% or higher concentrations of leaf extract and in 10% of bark extract, none of the embryos was hatched. The present review intends to provide a comprehensive assembly of studies that applied the zebrafish embryo model for the assessment of herbal toxicity. Toxicity Assessment of Herbal Medicine Using Zebrafish Embryos: A Systematic Review . Several morphological abnormalities such as the yolk retention, swim-bladder deficiency, pericardial oedema, and curved body shape were observed at 24 to 96 hpf in psoralen-treated embryos. Toxicity screening is conducted with animal and nonanimal models prior to clinical application. The developmental toxicity of EE were investigated using zebrafish embryo model. It has been reported 87% of genetic similarity exists between the zebrafish and human [11].

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